SOURCE: E. coli

PURITY: >98% by SDS-PAGE and HPLC analyses Endotoxin level is <0.1 ng per ug of G-CSF.

MOL. WEIGHT: 18.8 kDa

FORM: Lyophilized from 5 mM Sodium Citrate, pH 4.0.

RECONSTITUTION: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4oC for 1 week or -20oC for future use.

STORAGE CONDITIONS: The lyophilized G-CSF is best-stored desiccated below 0oC. Reconstituted G-CSF should be stored at working aliquots at -20oC.

DESCRIPTION: Granulocyte Colony Stimulating Factor (G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Human G-CSF is an 18.8 kDa protein containing 175 amino acid residues.

BIOLOGICAL ACTIVITY: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is < 0.1 ng/ml, corresponding to a Specific Activity of > 1 x 10⁸ units/mg.

NOTE:

FOR RESEARCH USE ONLY! NOT TO BE USED IN HUMANS.